Effect of platelet-rich plasma on limbal and amniotic stem cell viability and ‎proliferation: An in vitro study for ocular surface regeneration

Authors: Abdelkrim SALAMA, Ghyzlane EL Haddoumi ‎, Ilham Kandoussi ‎, Mohamed Elbelhadji ‎, Azeddine Ibrahimi ‎

DOI: 10.18231/j.ijceo.9413.1761818615

Keywords: Keywords: Platelet-Rich Plasma ; Limbal stem cells ; Amniotic stem cells ; Cell viability ; Cell proliferation ; ‎Tissue engineering ; Corneal regeneration.

Abstract: ABSTRACT Objective: To assess the influence of Platelet-Rich Plasma (PRP) on the survival and proliferation of rabbit ‎limbal and amniotic stem cells in vitro, with prospective implications for corneal regeneration. ‎ Materials and Methods: The study investigated the effects of PRP supplementation on stem cell cultures, ‎focusing on its influence on cell viability and proliferation. Quantitative analysis of cell viability and ‎proliferation was conducted, comparing PRP-treated groups with control groups through statistical analysis over ‎a span of five passages. ‎ Results: After 24 hours, limbal stem cells exposed to PRP exhibited a significantly higher viability rate of ‎‎90.60% compared to 83.40% in the control group (p = 0.001). Similarly, PRP-treated amniotic cells ‎demonstrated 86.40% viability, compared to 81.00% in controls (p = 0.002). After 48 hours, viability in PRP-‎treated limbal cells rose to 93.80%, compared to 86.60% in controls (p < 0.001), while amniotic cell viability ‎increased to 90.40% versus 85.80% in controls (p = 0.015). Proliferation rates also increased significantly in ‎both cell types with PRP treatment. Limbal cells showed a proliferation rate of 202.55%, compared to 195.61% ‎in controls (p < 0.001), and amniotic cells showed 173.43% versus 168.32% in controls (p < 0.001).‎ Conclusion: PRP significantly enhances both the viability and proliferation of rabbit limbal and amniotic stem ‎cells, highlighting its potential application in tissue engineering and corneal regeneration.‎ ‎